Determination of Genetic Diversity of Multidrug-Resistant Mycobacterium Tuberculosis Strains in Turkey Using 15 Locus MIRU-VNTR and Spoligotyping Methods
Yazarlar (6)
Doç. Dr. Burcu GÜRER GİRAY
Yalova Üniversitesi, Türkiye
Yozgat Bozok Üniversitesi, Türkiye
Doç. Dr. Didem ÖZGÜR
Kafkas Üniversitesi, Türkiye
Sağlık Bilimleri Üniversitesi, Türkiye
Mersin Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Pathogens and Global Health |
| Dergi ISSN | 2047-7724 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2023 |
| Cilt No | 117 |
| Sayı | 1 |
| Sayfalar | 85 / 91 |
| DOI Numarası | 10.1080/20477724.2022.2084807 |
| Makale Linki | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9848327/pdf/YPGH_117_2084807.pdf |
| Özet |
| Tuberculosis (TB) remains the leading cause of deaths from infectious disease worldwide. Nowadays, the tendency of complex (MTBC) to spread between continents due to uncontrolled migration movements shows that TB is a global health problem. The number of studies for the detection of MTBC strains' epidemiological features in areas with TB spread risk using molecular-based methods such as spoligotyping and Mycobacterial Interspersed Repetitive Unit (MIRU) Variable Number Tandem Repeats (VNTR) at the clonal level is insufficient. In this study, it was aimed to determine the phylogenetic relationships of MTBC strains at the species level by spoligotyping and 15 locus MIRU-VNTR (MIRU-VNTR) molecular methods of 96 multidrug-resistant (MDR) MTBC strains isolated from sputum samples of patients with a preliminary diagnosis of pulmonary TB or suspected contact history those sent to National Tuberculosis Reference Laboratory from the centers that are members of the Tuberculosis Laboratory Surveillance Network. The phylogenetic relationship between 96 MDR-TB strains was investigated with the combination of bead-based spoligotyping and MIRU-VNTR methods on the MAGPIX® Milliplex Map device. In this study, it was determined that the T1 family is more common in our country and LAM7-TUR family is less common than the Beijing family unlike other studies. It was determined that the strains in the same cluster had different locus profiles, and there was no transmission from the same clone in the clonal typing we performed with spoligotyping and MIRU-VNTR. |
| Anahtar Kelimeler |
| Mycobacterium tuberculosis complex | spoligotyping | MIRU-VNTR | MDR-TB | tuberculosis surveillance |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 1 |