UV curable methacrylated fumaric acid modified epoxy as a potential support for enzyme immobilization
Yazarlar (4)
M. Vezir Kahraman
Marmara Üniversitesi, Türkiye
Doç. Dr. Gülay BAYRAMOĞLU Marmara Üniversitesi, Türkiye
Nilhan Kayaman-Apohan
Marmara Üniversitesi, Türkiye
Atilla Güngör
Marmara Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Reactive and Functional Polymers (Q1)
Dergi ISSN 1381-5148 Dergi Bilgileri (2007)
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 02-2007
Kabul Tarihi – Yayınlanma Tarihi 01-02-2007
Cilt / Sayı / Sayfa 67 / 2 / 97–103 DOI 10.1016/j.reactfunctpolym.2006.09.005
Makale Linki http://linkinghub.elsevier.com/retrieve/pii/S1381514806001799
UAK Araştırma Alanları
Polimer Bilimi
Özet
The immobilization procedure of UV-curing coating is simple and causes less loss of enzymatic activity. UV-curable methacrylated/fumaric acid modified cycloaliphatic epoxide is here proposed as a rigid support material for covalent immobilization of α-amylase. The immobilized enzyme is analyzed in terms of bioactivity retention as a function of repeated use ability, pH, storage, as well as stability under various experimental conditions, taking starch as a substrate. The properties of immobilized enzyme were also compared with those of the free enzyme. The highest activity of free enzyme was obtained at pH 7.0 while this value was shifted to pH 7.5 for immobilized system. Optimum catalytic activity was observed at 30°C, for both free and immobilized enzyme; however, the immobilized enzyme had a higher activity than the free one. The immobilized enzyme that was used 35 times in 8h in repeated batch …
Anahtar Kelimeler
α-amylase | Cyclo aliphatic epoxide | Enzyme activity | Immobilization | UV-curing coating
Science Direct
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Web of Science 25
Scopus 33
Google Scholar 47
UV curable methacrylated fumaric acid modified epoxy as a potential support for enzyme immobilization